PhD Student (gn*) Testicular Extracellular Matrix

Developing a gel-based cell culture system to grow and differentiate Sertoli cells. Production of a recombinant integrin binding-competent laminin fragment that mimics basement membrane properties after its immobilisation to the cell culture gel system. Establishing Sertoli cell lines expressing N-cadherin mutants to study cell-cell interactions. Characterizing the differentiation state of Sertoli cells by collecting data from e.g. flow cytometry, video and fluorescence microscopy, immunoblotting, and transcriptome analysis. Analysing the cell biological, protein-chemical and molecular biological data on the Sertoli cells, also in co-culture with other testicular cell types. Collaborating with Prof. Dr. Stefan Schlatt's group (Center for Reproduction Medicine and Andrology) and other PIs of the CRC1748. Participating in the Integrated Research Training Group 'Reproduction.MS PhD-Training Centre in Translational Science'. Part of Research

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